The house is not only a biographical museum, it is also a museum of interiors and to a certain extent an art museum.

The more formal rooms of the house such as the drawing room and dining room are on the ground floor, while the more intimate rooms are on the upper floor. On the whole these are furnished with their original furniture, chandeliers, wall hangings, clocks, books and artworks, including numerous works by the artist – stained glass, oil paintings, decorative designs, drawings etc. The most valuable of these include the stained glass works – Vita Somnium Breve; Caritas; Faith, Hope, Love; portraits of the artist's wife – the so-called 'Florentine' portrait or In the Summer House and portraits of other people e.g. Zofia Minderowa (The Rose of Saron); landscapes such as The Vistula Near Niepołomice and Red Umbrella; designs e.g. Nature and Art (for the Palace of Art in Krakow) and Flowers (polychrome designs for the Treasury of Wawel Cathedral).

The house is complemented by a garden adjoining it on the southern side, which was carefully restored in 2003 to resemble as far as possible the original garden designed by Józef Mehoffer.

An illustrated guidebook for the exhibition is available either in Polish or English. The Józef Mehoffer House also runs its own educational and exhibition program, as well as hosting academic/artistic gatherings, and small concerts which are held in the drawing room or the garden.